Biochemical characterization for determination of genetic distances among different indigenous chickpea (Cicer arietinum L.) varieties of North-West Pakistan

Abstract Genetic distances among different chickpea varieties and evaluation of their free amino acid profiles were determined on the basis Sodium dodecyle sulphate polyacrylamide gels electrophoresis (SDS-PAGE). Total soluble proteins resolved 10% SDS Polyacrylamide gel. Low variability in tested was observed. Dendogram based electrophoretic data clustered genotypes into 2 groups. The results showed that average protein content all 26.01% within range 22.8% for Thal-2006 to 34.06% Sheenghar-2000 dry seed weight. On total Bittal-98, Dasht Sheen Ghar-2000, Karak-3 CM-98, Paidar -91 Fakhr-e-Thal, C-44, Balaksar KK-1showed similar concentrations contents each other but variation from rest varieties. Different separated changes molecular weights by means Dodecyl Sulphate Gel Electrophoresis Dasht, Ghar 100% similarity. Fakhr-e- Thal, KK-2 Chattan KC-98, KK-1 Lawaghar accessions. overall dendrogram high low level concentration acids varied 16 A significant difference both essential non-essential found cultivars. recorded 40.81 g/100 g while 59.18343 given highest C-44 followed KK-2, Fakhr E Tal lowest Cm-98, Paidar-91 Ghar-2000 respectively. Cultivars TAL-2006, maximum endogenous acids. In conclusion; broadening genetic pools breeding programs or search exotic characters, instance new disease resistance alleles, accession with similarity coefficients (Lawaghar Battal-98) may be utilized. Furthermore information acquired this study could used device a proficient approach intended at improving nutritional as well base food crop Pakistan.